Cannabidiolic acid synthase [CBDAS]

Cannabidiolic acid synthase (CBDAS) is an enzyme that catalyzes the conversion of cannabigerolic acid (CBGA) into cannabidiolic acid (CBDA), a precursor to cannabidiol (CBD). CBDAS is found in the trichomes of cannabis plants and is responsible for the biosynthesis of CBDA, which has potential therapeutic properties. The enzyme is a key player in the production of CBD, a compound that has gained popularity for its potential health benefits.

Enzyme Structure

• Cannabidiolic acid synthase is a single protein with a molecular mass of 74 kDa.
• Its amino acid sequence is partly homologous to oxidoreductases in other plants.
• CBDA synthase has four binding sites: two for FAD and two for the substrate.

Enzyme Function

• Catalyzes the production of cannabidiolate predominantly from cannabigerolate.
• Chemical reaction: cannabigerolate + O → cannabidiolate + H.
• Cannabinerolate can also be used as a substrate, but with lower efficiency.
• Optimum pH for CBDA synthase is 5.0.

References

• Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.
• Cannabidiolic-acid synthase is the chemotype-determining enzyme in fiber-type Cannabis sativa.
• Characterization and mechanism of the berberine bridge enzyme.
• Tobacco nectarin V is a flavin-containing berberine bridge enzyme-like protein with glucose oxidase activity.
• CBDAS – Cannabidiolic acid synthase precursor – Cannabis sativa (Hemp) – CBDAS gene & protein.

Cannabidiolic acid synthase Data Sources